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How to optimize the TLC of Carica papaya?
The main thing [WU2] of this experiment is to optimize the TLC protocol of aqueous extract of Carica papaya by using the right mobile solvent that will give the best resolution. The separation depends on the relative affinity of compounds towards stationary phase.
Why is silica gel used in TLC separation?
By understanding the relative polarities of the analgesic drugs, one could determine how the compounds will behave during the TLC separation. The stationary phase, silica gel in this experiment, is more polar, causing more polar components of a mixture to be more attracted to it.
What is a thin layer chromatography lab report?
Thin Layer Chromatography Lab Report. Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase.
Which is an example of a TLC chamber?
TLC chamber, is used for the development of TLC plate. The chamber maintains a uniform environment inside for proper development of spots. It also prevent the evaporation of solvents and keeps the process dust free. Mobile phase comprises of a solvent or solvent mixture.
Last Update: Oct 2021
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